Department of Plant Gene and Totipotency
Laboratory of Plant Molecular Biology
tkohchi -at- lif.kyoto-u.ac.jp
nishihama -at- lif.kyoto-u.ac.jp
Research in this laboratory focuses on the adaptive regulation of growth and development to environmental conditions using model photosynthetic organisms. Topics include mechanism of photomorphogenesis and meristem development. We study molecular mechanisms of light sensing by photoreceptors and downstream genetic network of signal transduction. Approaches by molecular genetics and comparative genomics are taken with Arabidopsis and liverwort (Marchantia polymorpha). In particular, the genetic analysis of Marchantia is unique and efficient as its phylogenetically basal position in land plant evolution, haploid-dominant life cycle, and simple transgenesis. Genome analysis of Marchantia is also conducted in this laboratory.
Laboratory of Molecular and Cellular Biology of Totipotency
fsato -at- lif.kyoto-u.ac.jp
tuendo -at- kais.kyoto-u.ac.jp
ifuku -at- kais.kyoto-u.ac.jp
Molecular and cellular biological studies on totipotency in plant cells have been carried out in this laboratory using in vitro cultured cells and transgenic plants. Especially, cell/organ differentiation from undifferentiated cells, functional differentiations, e.g. oxygen evolving complex in photosystem II, cyclic electron transfer and gene regulation in chloroplast, as well as molecular biology of secondary metabolism, such as biosynthesis of isoquinoline alkaloids in plant cells have been investigated to understand the totipotent functions in plant cells. Development of novel genetic engineering techniques such as differential RNAi, metabolic engineering and synthetic biology of secondary metabolism for industrial application have been also investigated.