Intramolecular FRET biosensors

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Targets Composition Name Lab PMID PubMed Year
Tyrosine kinase, substrate phosphorylation
EGFR CFP-SH2 (Shc)-Substrate (synthetic)-YFP EGFR-indicator Tsien 1E+07 11752449 2001
EGFR EGFR-CFP-PTB (Shc)-YFP FLAME Basitiaens 2E+07 15215236 2004
EGFR  -helix-YFP-CrkII-CFP Picchu-Z Matsuda 2E+07 15922734 2005
Insulin receptor CFP-Substrate (IRS1)-SH2 (p85)-YFP  Phocus Umezawa 1E+07 11875431 2002
Abl, EGFR YFP-CrkII-CFP  Picchu Matsuda 1E+07 11406630 2001
Abl, EGFR YPet-CrkII-EV-SECFP  PicchuEV Matsuda 2E+07 21976697 2011
Abl, EGFR CFP-CrkII-YFP Abl indicator Tsien 1E+07 11752449 2001
Abl Venus-CrkL-ECFP Pickles Ohba 2E+07 20670950 2010
Src TM (Cbp)-YFP-Substrate (Cbp)-SH2 (Csk)-CFP Chimera Okada 1E+07 11880369 2003
Src CFP-SH2 (Src)- Substrate (synthetic)-YFP Src indicator Tsien 1E+07 11752449 2008
Src CFP-SH2 (Src)- Substrate (synthetic)-YFP Src reporter Chien 2E+07 15846350 2005
Tyrosine kinase, conformational change
FAK CFP-FERM-Citrine-kinase-FAT CYFAK413 Schaller 2E+07 17967873 2008
FAK YFP-FERM-CFP-KD-FAT FERM-sensor Bunt  2E+07 19208768 2009
Serine/threonine kinase, substrate phosphorylation
PKA GFP-KID (PKA)-BFP ART Hagiwara 1E+07 10700148 2000
PKA CFP-[14-3-3]-Substrate (synthetic)-YFP  AKAR Tsien 1E+07 11752448 2001
PKA Ypet-FHA1-EV-Substrate-ECFP AKAR3-EV Matsuda 2E+07 21976697 2011
PKC YFP-PH and DEP (pleckstrin)-GFP KCP-2 Schultz 2E+07 16390103 2006
PKC YFP-PH and DEP (pleckstrin)-GFP KCAP-1 Schultz 2E+07 16390103 2006
PKC CFP-FHA2 (Rad53P)-Substrate(synthetic)-YFP  CKAR Newton 1E+07 12782683 2003
PKC C1-YPet-FHA1-EV-Substrate-ECFP Eevee-PKC Matsuda 2E+07 21976697 2011
AKT/PKB CFP-[14-3-3]-substrate-YFP Aktus Umezawa 1E+07 12773546 2003
AKT/PKB CFP-FHA2-substrate-YFP BKAR Newton 2E+07 15583002 2005
Akt/PKB PH-YPet-FHA1-EV-Substrate-ECFP Eevee-Akt Matsuda 2E+07 21976697 2011
PKD CFP-FHA2-substrate-YFP DKAR Newton 2E+07 17189263 2007
ERK CFP-FHA2-substrate-YFP-D domain Erkus Umezawa 2E+07 17261026 2007
ERK mRFP-WW-Long linker-substrate-D domain-EGFP EKAR Sbovoda 2E+07 19033456 2008
ERK Ypet-WW-EV-Substrate-D domain-ECFP EKAREV Matsuda 2E+07 21976697 2011
JNK ECFP-FHA1-JDP2-JDP2delta-Citrine JNKAR1 Zhang 2E+07 20212108 2010
JNK YPet-FHA1-EV-JDP2-JDP2delta-ECFP JNKAR1EV Matsuda 2E+07 21976697 2011
S6K YPet-FHA1-EV-Substrate-Turquiose Eevee-S6K Matsuda 2E+07 21976697 2011
RSK YPet-FHA1-EV-Substrate-ECFP Eevee-RSK Matsuda 2E+07 21976697 2011
MLCK, Rho kinase CFP-Myosin light chain-YFP CRCit Iino 2E+07 19033456 2005
Aurora B CFP-FHA2-substrate(Kif2 57-70)-YFP aurora B sensor Kapoor 2E+07 18463638 2008
Plk-1 CFP-FHA2-substrate(Myt1 421-432)-YFP Plk sensor Medema 2E+07 18615013 2008
SAP3K YFP-FHA1-MKK6 DN- DVD domain-CFP SAP3K activity reporter Saito 2E+07 19737916 2009
Serine/threonine phosphatases, substrate type
Calcineurin (PP2B) CFP-NFAT-Venus CaNAR1 Zhang 2E+07 18493642 2008
Serine/threonine kinase, conformational change
AKT/PKB GFP-AKT-YFPdark GFP-AKT-YFP Larijani 1E+07 12662152 2003
AKT/PKB PH (Akt)-YFP-AKT-CFP Akind Matsuda 2E+07 17079732 2007
PKCdelta CFP-PKC-YFP CY-PKCdelta  Blumberg 2E+07 15611119 2005
c-Raf YFP-cRaf-CFP Prin-cRaf Matsuda 2E+07 15711535 2005
B-Raf YFP-BRaf-CFP Prin-Braf Matsuda 2E+07 16858395 2006
ERK YFP-ERK-CFP Miu2 Matsuda 2E+07 16418172 2006
MLCK MLCK-GFP-BFP MLCK-FIP Chisholm 1E+07 11815633 2002
MLCK MLCK-CFP-YFP MLCK-FIP Chew 2E+07 20067998 2010
DAPK1 mTurquoise-DAPK1-Venus DAPK1(334)-F40 Schultz 2E+07 20067998 2011
CAMK2a mTurquoise-Camk2a-Venus Camk2a(314)-F40 Schultz 2E+07 20067998 2011
CAMK2a mTurquoise-Camk2a-Venus Camk2a-F63 Schultz 2E+07 20067998 2011
CAMK2a YFP-Camk2a-CFP Camui-alpha Hayashi 2E+07 20067998 2005
Histon methylation 
Histon H3-K9, K27 CFP-Chromodomain-histonH3-YFP K9, K27 reporter Ting 2E+07 15137760 2004
Histon H4-K5, K8 Venus-BRDT bromodomain-linker-HistoneH4-CFP Histac Yoshida 2E+07 19805290 2009
Small GTPases @
Ras, Rap1 YFP-Ras-Raf-CFP  Raichu-Ras, Rap1 Matsuda 1E+07 11429608 2001
Ras, Rap1 Ypet-Ras-EV-Raf-TurquoiseGL  RaichuEV-Ras Matsuda 2E+07 21976697 2011
Ral YFP-Ral-RalBP1-CFP Raichu-RalA, RalB Matsuda 2E+07 15034142 2004
RRas YFP-Rras-RalGDS-CFP Raichu-RRas Matsuda 2E+07 17344481 2007
RhoA YFP-PKN-CFP Raichu-RhoA Matsuda 1E+07 12860967 2003
Rac1 YFP-PAK-Rac1-CFP  Raichu-Rac1 Matsuda 1E+07 12192056 2002
Cdc42 YFP-PAK-Cdc42-CFP  Raichu-Cdc42 Matsuda 1E+07 12192056 2002
Rac, Cdc42 YPet-PAK-Rac1/Cdc42-CFP  Raichu-Rac Wang 2E+07 18799748 2008
Rac, Cdc42 YPet-PAK-EV-Rac1-CFP  Raichu-Rac Matsuda 2E+07 21976697 2011
TC10 YFP-POSH-TC10-CFP Raichu-TC10 Matsuda 2E+07 16950130 2006
RhoA YFP-Rhotekin-CFP Raichu-RBD Matsuda 1E+07 12860967 2003
Rac, Cdc42 YFP-PAK-CFP Raichu-CRIB Matsuda 1E+07 12192056 2002
Rab5 YFP-EEA1-CFP-Rab5 Raichu-Rab5 Matsuda 2E+07 18385674 2008
RhoA RBD-CFP-YFP-RhoA RhoA biosensor Hahn 2E+07 16547516 2006
Cdc42 Cdc42-YFP-GBD-ACV-CFP GEF sensor Rosen 1E+07 12680752 2003
N-WASP/Cdc42 CFP-N-WASP-YFP N-WASP-BS Condeelis 2E+07 15084285 2004
GPCR
PTHR PTHR-CFP-PTHR-YFP PTHR-cam Lohse 1E+07 12808462 2003
2AAR 2AAR-CFP-2AAR-YFP 2AAR-cam Lohse 1E+07 12808462 2003
Lipids
PIP3 CFP-PH (GRP)-YFP-membrane anchor Fllip/Pippi-PIP3 Umezawa 1E+07 14528311 2003
PIP2 and PIP3 CFP-ActA-YFP-membrane anchor CAY Cicchetti 1E+07 14967034 2003
PIP3, PI(3,4)P2 CFP-PH(Akt)-Pseudoligand-YFP InPAkt Zhang 2E+07 16214892 2005
PI(4,5)P2 CFP-PH (GRP)-YFP-membrane anchor CYPHR Newton 1E+07 12782683 2003
PI(4,5)P2 CFP-PH (PLCd)-YFP-membrane anchor Pippi-PI(4,5)P2 Matsuda 2E+07 18685081 2008
PI(3,4)P2 CFP-PH (TAPP1)-YFP-membrane anchor Pippi-PI(3,4)P2 Matsuda 2E+07 17079732 2007
IP3 CFP-IP3 receptor-YFP LIBRA Tojyo 2E+07 15272011 2004
IP3 CFP-IP3 receptor-YFP fretino Umezawa 2E+07 16053285 2005
IP3 CFP-IP3 receptor-YFP FIRE Mignery 2E+07 16249182 2006
Diacylglycerol CFP-C1 (PKC)-YFP DAGR Newton 1E+07 12782683 2003
Diacylglycerol CFP-CRD (PKC)-YFP-membrane anchor Daglas Umezawa 2E+07 16990811 2006
Diacylglycerol CFP-CRD (PKC)-YFP-membrane anchor DIGDA Matsuda 2E+07 18685081 2008
PI(4)P CFP-PH (GRP)-YFP-membrane anchor Pippi-PI(4)P Matsuda 2E+07 18685081 2008
Phosphatidic acid CFP-PA binding doman-YFP-membrane anchor Pii Matsuda 2E+07 20826779 2010
Ions
calcium  CFP-Calmodulin-M13-YFP cameleon Tsien 9E+06 9278050 1997
calcium CFP-Kringle domain (apoK1)-YFP apoK1-er Graier 2E+07 16368693 2006
calcium BGFP-MLCK-RGFP FIP-CA, CB Persechini 9E+06 9148946 1997
chloride CFP-linker-YFP clomeleon Augustine 1E+07 11055428 2000
Zn Cerulean-Atox1-WD4-Citrine eCALWY-1 Merkx 2E+07 19718032 2009
Steroids
androgen CFP-androgen binding domain-coactivator-YFP ficaro Umezawa 2E+07 16555356 2006
estrogen/androgen CFP-estrogen or androgen receptor-YFP CEY, CAY Lannigan 2E+07 15985367 2005
retinoic Acid CFP-RAR-YFP GEPRAs Miyawaki 2E+07 23563268 2013
Amino acids
glutamate CFP-glutamate binding domain-YFP FLIPE Frommer 2E+07 15939876 2005
Sugars
glucose MglB(1-12)-ECFP-MglB (13-304)-linker-Citrine FLII12Pglu Frommer 2E+07 18177733 2007
sugar CFP-sugar binding domain-YFP nanosensors Frommer 1E+07 15667328 2005
lactate mTFP-LidR-Venus Laconic Barros 1E+07 23469056 2013
Cyclic nucleotides
cGMP YFP-PKG-CFP CGY Umezawa 1E+07 11140757 2000
cGMP CFP-GKI-YFP cygnet Dostmann 1E+07 11226257 2001
cGMP YFP-phosphodiesterase-CFP pGES-DE2 Lohse 2E+07 16369548 2006
cAMP CFP-Epac-YFP CFP-Epac-YFP Jalink 2E+07 15550931 2004
cAMP CFP-Epac-YFP ICUE Zhang 2E+07 15545605 2004
cAMP CFP-Epac-YFP Epac2-camps Lohse 2E+07 15231839 2004
cAMP ECFP-cAMP-binding domain B (mammalian PKA RII beta-subunit)-YFP cAMP FRET sensor Parent 2E+07 19808889 2009
ATP mseCFP-eSubunit(bacterial FoF1-ATP synthase)-cp173mVenus ATeam Noji 2E+07 19720993 2009
Proteases
Caspase3&6 CFP-SGDEVD-YFP-RSRAQASVEID-mRFP CFP-c3-YFP-c6-mRFP He 2E+07 16683263 2006
Caspase8 YFP-Bid-CFP CFP-c3-YFP-c6-mRFP Taira 1E+07 12409609 2002
Caspase3, 9 ECFP-cleavage site (DEVD or VEID) -Venus SCAT3, 9 Miura 1E+07 12527749 2003
Caspase3 BFP-DEVD-GFP BFP-DEVD-GFP Prehn 1E+07 11964393 2002
Caspase3 CFP-DEVD-YFP CFP-DEVD-YFP Tavare  1E+07 11256610 2000
Caspase3 EYFP–DEVD–EGFP EYFP–DEVD–EGFP Bastiaens 1E+07 11175733 2001
Caspase3 EGFP–DEVD–EBFP EGFP–DEVD–EBFP Luo 1E+07 9518501 1998
Caspase3 mAmetrine–DEVD–tdTomato, mCitrine–DEVD–mTFP1 mAmetrine–DEVD–tdTomato, mCitrine–DEVD–mTFP1 Campbell 2E+07 18425137 2008
MMP activity Igk-Chain-YFP-MSS-CFP-TM (PDGFR) pDisplay Luo 2E+07 17187878 2007
MMP activity 6His-YFP-MSS-CFP pDisplay Luo 2E+07 17187878 2007
MT1-MMP  Ypet-[MMP substrate]-CFP  MT1-MMP FRET@biosensor Wang 2E+07 18441011 2008
MT1-MMP  mCherry-MT1-[MMP substrate]-mOrange2 MT1-MMP FRET@biosensor Wang 2E+07 20197470 2010
m-caplain activity EYFP-mCalpain cleavage site-ECFP-PDZ1-1 targeting seq/ YSCS Edelman 1E+07 10688895 2000
Miscellaneous
K+ channel Kv potassium channel-CFP-YFP VSFP1 Knoepfel 1E+07 11454036 2001
HIV Rev YFP-Rev peptide-CFP YRFnC-11ad Kobatake 2E+07 16013840 2005
Presenilin (PS) GFP-PS1-RFP GFP-PS1-RFP Luo 2E+07 19924286 2009
Plasma membrane Ca(2+) pump BFP-plasma membrane Ca2  pump-GFP BFP-PMCA-GFP Adamo 2E+07 17901055 2007
LuxP CFP-LuxP (BAI-2 receptor)-YFP CLPY Sayre 2E+07 17352493 2007
MyosinII YFP−myosin(motor domain)−CFP YFP-myosin-CFP Bagshaw 2E+07 16939200 2006
MyosinII GFP−myosin(motor domain)−BFP GFP-myosin-BFP Bagshaw 2E+07 16939200 2006
MyosinII His-GFP−S1dC (motor domain of MyosinII)−BFP chimaera Sutoh 1E+07 9845076 1997
MyosinII His-BFP−S1dC−GFP chimaera Sutoh 1E+07 9845076 1997
REDOX Cerulean-Yap1 c-CRD-Citrine Redoxfluor Sakai 2E+07 20498274 2010
REDOX ECFP-RL7-EYFP redox biosensor Gaskins 2E+07 21606117 2011