講演タイトル
「オルガネラ特異的同位体標識と検出によるオルガネラ間脂質流動の追跡」 ポスター🔗
講演者
植松 真章 博士（コーネル大学・M.D.,Ph.D.）
開催日時・場所
2025年11月20日(木) 16:15～17:15
京都大学 医学・生命科学総合研究棟(G棟)2階セミナー室A
要旨
Lipids are essential in defining organelle boundaries that enable compartmentalized biological reactions. Although continuous lipid synthesis, degradation, and transport are important for organelle homeostasis, tracking these dynamics—especially inter-organelle lipid transport—is difficult because biochemical isolation of organelle lipids compromises spatial resolution and quantification. Alternative approaches—such as chemically installing fluorophores or small chemical handles, or expressing genetically encoded lipid-binding probes—often perturb lipid function and localization. To overcome these limitations, I developed a minimally perturbative method to measure inter-organelle transport of phosphatidylcholine (PC), the most abundant phospholipid in mammalian cells, with high spatiotemporal resolution. Using phospholipase D (PLD), I established an organelle-specific labeling system that converts endogenous PC into deuterated PC. PLD was engineered to be light-activatable, enabling precise pulse labeling. When combined with a second, drug-activatable PLD, the platform enables detection of the transported, labeled PC on another organelle, quantifying PC flux between any desired pair of organelles. It can also be applied to absolute quantification and turnover measurements of organelle PC. Finally, I introduce AI-driven protein design to extend the approach to other lipid classes beyond PC by tuning PLD substrate preferences. Together, these advances are expected to enable a quantitative, systems-level understanding of intracellular lipid transport.
連絡先
統合生命科学専攻 遺伝機構学講座 細胞周期学分野
生命情報解析教育センター 【CeLiSIS】
青木 一洋（aoki.kazuhiro.6v@kyoto-u.ac.jp）
