Title
「Mapping the Chloroplast: A Protein Localization Atlas and Protein-Protein
Interaction Network」 ポスター🔗
Lecturer
Dr. Lianyong Wang（Northeastern University, USA）
Date, Time & Venue
September 16 (Tue), 16:00-17:00 Seminar Room (2), Agriculture and Biostudies Building ,
North Campus
Abstract
Chloroplasts are crucial to photosynthesis and metabolism, yet the spatial
organization of their protein complexes remains incompletely understood. Here, we
systematically determined the subcellular localization of over 1,000 chloroplastassociated
proteins in Chlamydomonas reinhardtii using fluorescent tagging,
identifying novel punctate structures and their components. We also identified 4,837
high-confidence protein-protein interactions using affinity purification-mass
spectrometry, illuminating the organizations of protein complexes in chloroplast
pathways, such as the Calvin-Benson cycle, ATP synthesis, and photosystem repair. By
combing with the structural interaction model AlphaFold 3, we predicted direct
interactions. We hope that this dataset will serve as a useful resource for exploring
chloroplast functions.
Reference: Wang L et al. Cell 186:3499–3518 (2023)
Contact
Division of Integrated Life Science, Laboratory of applied molecular microbiology
Takashi YAMANO（tyamano@lif.kyoto-u.ac.jp）
