講演タイトル
「The biophysical properties of the cytoplasm in yeast cells: Two case studies on dormancy and excessive cell growth」 ポスター🔗
講演者
Dr. Keiichiro Sakai(Amy E. Ikui lab, Brooklyn College at The City University of New York, Brooklyn, U.S.A.)
開催日時・場所
2024年11月25日(月) 13:30~15:00
京都大学 医学・生命科学総合研究棟(G棟)2階、セミナー室C/D
要旨
The cytoplasm is a complex and crowded environment, which influences a wide range of cellular processes such as biochemical reactions and protein folding. Recent studies have reported that cells adjust the biophysical properties of the cytoplasm in response to environmental changes for homeostasis and adaptation. We used yeast cells to investigate how the cytoplasmic properties change and what the molecular mechanisms underlie the two phenomena; dormancy and excessive cell growth. Dormancy is a physiological state in which cells stop growing and proliferating reversibly under harsh environmental conditions. Also, excessive cell growth impairs cell-cycle progression and cell signaling in cells that have grown too large. To visualize cytoplasmic fluidity in these cellular states, we used genetically encoded multimeric nanoparticles (GEMs), which self-assemble and form a spherical particle of 40 nm diameter. By tracking GEMs, we found that cytoplasmic fluidity is reduced during dormancy and increased during excessive cell growth. In this seminar, I will discuss the biophysical properties of the cytoplasm during dormancy and excessive cell growth and the molecular pathway involved in these processes.
連絡先
細胞周期学分野 青木一洋(Kazuhiro Aoki)
aoki.kazuhiro.6v@kyoto-u.ac.jp