matsumoto.tomohiro.7s -at- kyoto-u.ac.jp
furuya.kanji.5a -at- kyoto-u.ac.jp
The spindle checkpoint, our major research subject, is a surveillance mechanism to regulate cellular apparatus for compliance with this rule. It is a unique negative feedback that converts/amplifies a physical signal sensed by kinetochores (attachment of the spindle and/or tension) and regulates the timing of the sister chromatid separation. Mad2, a signal carrier of this feedback, plays a vital role in the spindle checkpoint. It is specifically localized at unattached kinetochores that are the origin of the checkpoint signal. Mad2 targets CDC20 and inhibits its activity to promote sister chromatid separation. We study Mad2, a central player of the spindle checkpoint, to reveal mechanisms, which regulate the activity of Mad2.